The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of skin.
Rahn, E., Thier, K., Petermann, P., Knebel-Mörsdorf, D. Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1. J. Vis. Exp. (102), e53046, doi:10.3791/53046 (2015).