このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
L-ロイシンメチルエステルを使用した小脳顆粒細胞培養物からのミクログリアの選択的枯渇
章
- 00:05標題
- 01:58Culturing the Cerebellar Granule Cells
- 04:47Selectively Depleting the Microglia at 6 Days In Vitro
- 06:35Results: Characterization of LME Treatment on CGCs and Neurons and Astrocytes in CGC Cultures
- 07:51Conclusion
Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.
