このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
배아의 형광 라벨의 DNA 특정 얼룩 메틸 녹색의 응용 프로그램
13,923 Views
•
05:59 min
•
May 02, 2015
DOI:
概要
Automatically generated
A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.
Read Article
Cite this Article
Prieto, D., Aparicio, G., Machado, M., Zolessi, F. R. Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos. J. Vis. Exp. (99), e52769, doi:10.3791/52769 (2015).
Copy