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胚の蛍光標識でDNA特異的染色メチルグリーンの応用
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発生生物学
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JoVE Journal 発生生物学
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
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胚の蛍光標識でDNA特異的染色メチルグリーンの応用

13,923 Views

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05:59 min

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May 02, 2015

DOI:

10.3791/52769-v

DOI:
10.3791/52769-v

•

05:59 min
May 02, 2015

•

7 Views
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1Institut Pasteur de Montevideo, 2Sección Biología Celular, Facultad de Ciencias,Universidad de la República

概要

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A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.

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Cite this Article

Prieto, D., Aparicio, G., Machado, M., Zolessi, F. R. Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos. J. Vis. Exp. (99), e52769, doi:10.3791/52769 (2015).

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