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Anwendung der DNA-spezifisches Färbemittel, Methyl Green in der Fluoreszenzmarkierung von Embryos
JoVE Journal
発生生物学
Author Produced
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JoVE Journal 発生生物学
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

Anwendung der DNA-spezifisches Färbemittel, Methyl Green in der Fluoreszenzmarkierung von Embryos

DOI:

05:59 min

May 02, 2015

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  • 00:00標題
  • 01:34Dye Preparation
  • 02:36Whole-mount Embryo Labeling
  • 03:39Confocal Imaging
  • 04:19Representative Results
  • 05:15Conclusion

概要

自動翻訳

A method for fluorescent staining of fixed biological material with the specific DNA label methyl green is described. Methyl green is used in a diluted aqueous solution and is very resistant to photobleaching. Its far-red emission allows for deep specimen imaging, making it particularly adequate for whole embryos.

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