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タイムラプス明実体顕微鏡でゼブラフィッシュ幼虫における組織修復をキャプチャ
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January 31, 2015
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We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
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Lisse, T. S., Brochu, E. A., Rieger, S. Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy. J. Vis. Exp. (95), e52654, doi:10.3791/52654 (2015).
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