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プラークアッセイを通じてウイルス濃度の測定:伝統と新しいオーバーレイシステムの使用
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

プラークアッセイを通じてウイルス濃度の測定:伝統と新しいオーバーレイシステムの使用

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09:28 min

November 04, 2014

DOI:

09:28 min
November 04, 2014

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概要

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Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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