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High-throughput Assay per Fenotipo<em> Salmonella enterica</em> Typhimurium Associazione, Invasion, e replica nei macrofagi
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11:10 min
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August 11, 2014
DOI:
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine,Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine,University of California, Davis
概要
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Un test high-throughput per fenotipo Salmonella in vitro o di altra associazione batterica, l'invasione, e la replica in fagociti con capacità high-throughput è stato sviluppato. Il metodo è stato impiegato per valutare Salmonella gene knockout ceppi mutanti per il loro coinvolgimento in interazioni ospite-patogeno.
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Wu, J., Pugh, R., Laughlin, R. C., Andrews-Polymenis, H., McClelland, M., Bäumler, A. J., Adams, L. G. High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages. J. Vis. Exp. (90), e51759, doi:10.3791/51759 (2014).
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