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Utilizzo di proteine ​​fluorescenti per monitorare Glycosome Dynamics in Tripanosoma africano
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JoVE Journal 免疫学と感染
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
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Utilizzo di proteine ​​fluorescenti per monitorare Glycosome Dynamics in Tripanosoma africano

8,872 Views

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10:04 min

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August 19, 2014

DOI:

10.3791/51647-v

DOI:
10.3791/51647-v

•

10:04 min
August 19, 2014

•

6 Views
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1Department of Genetics and Biochemistry,Clemson University Eukaryotic Pathogens Innovation Center

概要

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Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

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Fluorescent ProteinsGlycosome DynamicsTrypanosoma BruceiHuman African TrypanosomiasisNaganaPeroxisomesGlycolysisFluorescence MicroscopyPTS2-eYFPFlow CytometryOrganelle Composition

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Cite this Article

Bauer, S., Conlon, M., Morris, M. Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome. J. Vis. Exp. (90), e51647, doi:10.3791/51647 (2014).

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