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免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Please note that all translations are automatically generated. Click here for the English version.

免疫突触由双色时间选通受激发射损耗（STED）纳米显微可视化

77,780 Views

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

概要

Automatically generated

在这里，我们示出了协议，用于成像由两色STED纳米显微NK细胞扼要重述在玻璃上的细胞毒免疫突触。使用这种方法，我们获得的突触蛋白和细胞骨架的亚100纳米分辨率。

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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