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Visualizzazione della immunologica Synapse da Dual Color Time-gated emissione stimolata Depletion (STED) Nanoscopia
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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Visualizzazione della immunologica Synapse da Dual Color Time-gated emissione stimolata Depletion (STED) Nanoscopia

77,780 Views

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

概要

Automatically generated

Qui illustriamo il protocollo per l'imaging a due colori STED nanoscopia il citotossica sinapsi immunitaria delle cellule NK ricapitolato su vetro. Usando questo metodo si ottiene nm risoluzione sub-100 di proteine ​​sinaptiche e il citoscheletro.

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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