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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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JoVE Journal 免疫学と感染
Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

5,923 Views

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09:10 min

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December 16, 2013

DOI:

10.3791/50952-v

DOI:
10.3791/50952-v

•

09:10 min
December 16, 2013

•

1 Views
,
1School of Immunity and Infection, IBR-MRC Centre for Immune Regulation, College of Medical and Dental Sciences,University of Birmingham, 2Centre for Cardiovascular Sciences,University of Edinburgh

概要

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Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.

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Cite this Article

Benezech, C., Caamano, J. H. Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin. J. Vis. Exp. (82), e50952, doi:10.3791/50952 (2013).

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