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生成和动脉病变的小鼠的三维定量使用光学投影层析成像
JoVE Journal
医学
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JoVE Journal 医学
Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
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生成和动脉病变的小鼠的三维定量使用光学投影层析成像

DOI:
10.3791/50627-v

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11:45 min

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May 26, 2015

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, , , , , , ,
1University/ BHF Centre for Cardiovascular Science, University of Edinburgh,The Queen’s Medical Research Institute

章

  • 00:05標題
  • 01:50Mouse Femoral Artery Lesion Surgery
  • 05:12Analyzing Arterial Lesion by Optical Projection Tomography (OPT)
  • 07:40Image Reconstruction and Analysis
  • 09:10Optical Projection Tomographs of Lesioned Femoral Arteries and Aortic Arch
  • 10:51Conclusion

概要

自動翻訳

自動翻訳

Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

タグ

Optical Projection Tomography3D QuantitationArterial LesionsMiceAtherosclerosisNeointimal LesionsWire-insertionLigationApoE-deficientHistologyImmunohistochemistryVascular Remodeling

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