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免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片
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JoVE Journal 神経科学
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

16,814 Views

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09:11 min

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November 14, 2016

DOI:

10.3791/50425-v

DOI:
10.3791/50425-v

•

09:11 min
November 14, 2016

•

10 Views
, , , , ,
1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine,Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research,Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit,Karolinska Institutet

概要

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We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

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ImmunohistochemistryRat Central Nervous SystemPeripheral Lymph NodeProtein LocalizationParaffin-embeddingAntigen RetrievalAntibodiesSignal DetectionLight Microscopy

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Cite this Article

Adzemovic, M. Z., Zeitelhofer, M., Leisser, M., Köck, U., Kury, A., Olsson, T. Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections. J. Vis. Exp. (117), e50425, doi:10.3791/50425 (2016).

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