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De procéder au lavage vaginal, coloration au cristal violet et l'évaluation cytologique vaginal pour Souris cycle œstral Staging identification
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09:01 min
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September 15, 2012
DOI:
1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
概要
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Ici, nous décrivons comment identifier le stade de la reproduction murin (proestrus, oestrus, metestrus ou diestrus) par simple, non invasive collecte et l'évaluation cytologique des échantillons frottis vaginaux. En outre, nous décrivons comment la cytologie vaginale reflète les taux circulants hormonaux qui sous-tendent la transition dans le cycle de reproduction murin.
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Cite this Article
McLean, A. C., Valenzuela, N., Fai, S., Bennett, S. A. Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification. J. Vis. Exp. (67), e4389, doi:10.3791/4389 (2012).
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