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基于miRNA的质粒在发展中的神经管和表型的评估DiI荧光注射开卷准备在卵的电
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JoVE Journal 神経科学
In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
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基于miRNA的质粒在发展中的神经管和表型的评估DiI荧光注射开卷准备在卵的电

16,327 Views

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12:41 min

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October 16, 2012

DOI:

10.3791/4384-v

DOI:
10.3791/4384-v

•

12:41 min
October 16, 2012

•

2 Views
,
1Institute of Molecular Life Sciences,University of Zurich

概要

Automatically generated

可以下调基因的表达在神经管的方法是，在特定的细胞类型，可跟踪的方式进行描述。我们演示了如何<em在OVO</em>电的小分子RNA的质粒，引起时空控制的RNA干扰可用于研究在发展中的神经管连合轴突导向。

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Wilson, N. H., Stoeckli, E. T. In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations. J. Vis. Exp. (68), e4384, doi:10.3791/4384 (2012).

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