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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法

JoVE Journal
生物学

This content is Free Access.

JoVE Journal 生物学

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法

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22:49 min

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December 13, 2012

DOI:

DOI:

•

22:49 min

December 13, 2012

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17 Views

Joseph D. Tario Jr.¹, Kristen Humphrey¹, Andrew D. Bantly², Katharine A. Muirhead³, Jonni S. Moore⁴, Paul K. Wallace¹

¹Department of Flow and Image Cytometry,Roswell Park Cancer Institute, ²Flow Cytometry & Cell Sorting Resource Laboratory,University of Pennsylvania , ³SciGro, Inc., ⁴Department of Pathology and Laboratory Medicine,University of Pennsylvania

概要

成功地利用监测免疫细胞的功能和增殖的细胞追踪染料涉及的几个关键步骤。我们描述的方法为：1）获得明亮，均匀，重现性好标签的膜染料; 2）选择荧光染料和数据采集条件; 3）选择一个模型来量化细胞增殖的基础上染料稀释。

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