ジャーナル
/
生物学
/
細胞侵襲DISC1タンパク質種の生成、精製とキャラクタリゼーション
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
Please note that all translations are automatically generated. Click here for the English version.

細胞侵襲DISC1タンパク質種の生成、精製とキャラクタリゼーション

13,516 Views

•

14:33 min

•

August 30, 2012

DOI:

10.3791/4132-v

DOI:
10.3791/4132-v

•

14:33 min
August 30, 2012

•

1 Views
, , , ,
1Department of Neuropathology,Medical School Düsseldorf, Germany, 2Center of Behavioral Neuroscience,University of Düsseldorf

概要

Automatically generated

細胞培養液からの細胞内、細胞質の全長DISC1タンパク質のアグレソームのとで標識し、多量換えDISC1タンパク質断片の生成、精製および細胞浸潤<em> E大腸菌の</em>記載されています。細胞の浸潤は、細胞培養とニューロンの受容細胞のために示されて<em生体内で></em> stereotactical脳接種後。

Explore More Videos

Protein AggregationChronic Degenerative Brain ConditionsNeurodegenerative DiseasesAlzheimer’s DiseaseTauParkinson’s DiseaseAlpha-synucleinHuntington’s DiseasePolyglutamineHuntingtinProteostasisMisfolded ProteinsSporadic FormsFamilial FormsSchizophreniaCognitive DeficitDisrupted-in-schizophrenia 1 (DISC1)Scottish FamilyChronic Mental DiseaseInsoluble AggregatesSMRI CCSarkosyl-insoluble DISC1 ImmunoreactivityBiochemical FractionationDisease-associated Polymorphism S704CDISC1 Aggresomes

Read Article

Cite this Article

Bader, V., Ottis, P., Pum, M., Huston, J. P., Korth, C. Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species. J. Vis. Exp. (66), e4132, doi:10.3791/4132 (2012).

Download .ris file

Copy

Share Video

.

Copy