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侵入细胞DISC1蛋白种类的产生，纯化及表征
JoVE Journal
生物学
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JoVE Journal 生物学
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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侵入细胞DISC1蛋白种类的产生，纯化及表征

DOI:
10.3791/4132-v

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14:33 min

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August 30, 2012

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, , , ,
1Department of Neuropathology,Medical School Düsseldorf, Germany, 2Center of Behavioral Neuroscience,University of Düsseldorf

章

  • 00:05標題
  • 02:47Preparation of Aggresome Donor Cells: Transfection of mRFP- or eGFP-tagged Human Full-length DISC1
  • 03:50Aggresome Purification from Transfected Donor Cells
  • 06:43Treatment of Recipient SH-SYBY Cells with mRFP-DISC1 Aggresomes
  • 08:13Labeling of Recombinant DISC1(598-785) with DyLight594
  • 10:44Treatment of Recipient SH-SY5Y Cells with Labeled Recombinant DISC1(598-785) Protein
  • 12:00Representative Invasion Assay Results
  • 13:30Conclusion

概要

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自動翻訳

的产生，纯化和细胞浸润的细胞，胞浆全长DISC1蛋白aggresomes从细胞培养和标记，多聚重组DISC1蛋白片段中<em> E.大肠杆菌</em>进行说明。为受体细胞在细胞培养和神经元细胞的侵袭<em在体内</em>后脑接种stereotactical。

タグ

Protein AggregationChronic Degenerative Brain ConditionsNeurodegenerative DiseasesAlzheimer’s DiseaseTauParkinson’s DiseaseAlpha-synucleinHuntington’s DiseasePolyglutamineHuntingtinProteostasisMisfolded ProteinsSporadic FormsFamilial FormsSchizophreniaCognitive DeficitDisrupted-in-schizophrenia 1 (DISC1)Scottish FamilyChronic Mental DiseaseInsoluble AggregatesSMRI CCSarkosyl-insoluble DISC1 ImmunoreactivityBiochemical FractionationDisease-associated Polymorphism S704CDISC1 Aggresomes

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