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RIP-チップを用いた細胞抽出物からmRNAを、マイクロRNAとリボ核タンパク質複合体のタンパク質成分の分離と​​同定するための方法
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生物学
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JoVE Journal 生物学
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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RIP-チップを用いた細胞抽出物からmRNAを、マイクロRNAとリボ核タンパク質複合体のタンパク質成分の分離と​​同定するための方法

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13:34 min

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September 29, 2012

DOI:

10.3791/3851-v

DOI:
10.3791/3851-v

•

13:34 min
September 29, 2012

•

1 Views
, , , , ,
1Molecular Microbiology and Immunology,University of Missouri, 2外科,University of Missouri, 3Child Health,University of Missouri

概要

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RIP-チップを介したRNA関連した複合体を単離および同定するためのステップバイステップのプロトコル。

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IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions

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Cite this Article

Dahm, G. M., Gubin, M. M., Magee, J. D., Techasintana, P., Calaluce, R., Atasoy, U. Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip. J. Vis. Exp. (67), e3851, doi:10.3791/3851 (2012).

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