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Método para el aislamiento e identificación de mRNAs, microRNAs y componentes proteicos de ribonucleoprotein complejos de extractos celulares usando RIP-Chip
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September 29, 2012
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概要
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Un paso a paso el protocolo para aislar e identificar los complejos de ARN asociados a través de RIP-Chip.
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IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions
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Cite this Article
Dahm, G. M., Gubin, M. M., Magee, J. D., Techasintana, P., Calaluce, R., Atasoy, U. Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip. J. Vis. Exp. (67), e3851, doi:10.3791/3851 (2012).
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