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RIP 칩을 사용하여 세포 추출물에서 mRNAs, microRNAs 및 Ribonucleoprotein 단지의 단백질 구성 요소의 분리 및 확인에 방법
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生物学
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JoVE Journal 生物学
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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RIP 칩을 사용하여 세포 추출물에서 mRNAs, microRNAs 및 Ribonucleoprotein 단지의 단백질 구성 요소의 분리 및 확인에 방법

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13:34 min

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September 29, 2012

DOI:

10.3791/3851-v

DOI:
10.3791/3851-v

•

13:34 min
September 29, 2012

•

1 Views
, , , , ,
1Molecular Microbiology and Immunology,University of Missouri, 2外科,University of Missouri, 3Child Health,University of Missouri

概要

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RIP 칩을 통해 RNA 관련 단지를 분리하고 식별 단계 프로토콜로 단계.

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IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions

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Cite this Article

Dahm, G. M., Gubin, M. M., Magee, J. D., Techasintana, P., Calaluce, R., Atasoy, U. Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip. J. Vis. Exp. (67), e3851, doi:10.3791/3851 (2012).

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