このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
光谱共焦成像的荧光标记的尼古丁受体敲在慢性尼古丁政府小鼠
13,371 Views
•
08:47 min
•
February 10, 2012
DOI:
概要
Automatically generated
我们已经开发出一种量化烟碱型乙酰胆碱受体亚地区的中枢神经系统神经元的具体亚型内的变化，更好地了解使用爆震的方法和光谱用荧光蛋白标记的受体相结合的办法，包括对尼古丁成瘾的机制的新技术共聚焦成像。
Explore More Videos
Spectral Confocal ImagingFluorescently Tagged Nicotinic ReceptorsKnock-in MiceChronic Nicotine AdministrationLigand-gated Ion ChannelsCentral Nervous System (CNS)Addiction To NicotineIon Channel DistributionChronic Nicotine ExposureNicotinic Acetylcholine Receptors (nAChRs)Glutamatergic GluN1GluA1 ChannelsSensitization To Addictive DrugsMap And Quantify DistributionExpression PatternsSpecific Ion ChannelsMechanisms Of AddictionBrain Region-specific EffectsRadioactive LigandsLow Spatial ResolutionLigand-gated Ion Channels In NeuronsGenetically Encoded Fluorescent Reporters
Read Article
Cite this Article
Renda, A., Nashmi, R. Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration. J. Vis. Exp. (60), e3516, doi:10.3791/3516 (2012).
Copy