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在体内肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注
JoVE Journal
生物学
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JoVE Journal 生物学
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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在体内肝细胞的分离纯化的肝细胞内吞其次是肝脏灌注

18,730 Views

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12:35 min

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November 10, 2011

DOI:

10.3791/3138-v

DOI:
10.3791/3138-v

•

12:35 min
November 10, 2011

•

7 Views
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1Department of Biochemistry,University of Nebraska, Lincoln

概要

Automatically generated

从动物获得的无细胞系存在的主要细胞，肝窦内皮细胞（秒）的研究必须执行。这种方法依赖于肝的消化和差速离心SEC随后的培养和实验净化。

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Cite this Article

Gopalakrishnan, S., Harris, E. N. In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. J. Vis. Exp. (57), e3138, doi:10.3791/3138 (2011).

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