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基因小鼠胚胎干细胞的基础含量为心源性诱导效率量化
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April 22, 2011
DOI:
1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine,Veterans Administration TVHS
概要
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我们描述了一个小鼠胚胎干细胞实验，以确定Wnt信号/β- catenin和BMP期间心感应信号激活的关键时间窗。该方法提供了一个标准化的平台，可靠的量化心源性效率，它是适用于其他细胞谱系的研究。
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Cite this Article
Ao, A., Williams, C. H., Hao, J., Hong, C. C. Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency. J. Vis. Exp. (50), e2656, doi:10.3791/2656 (2011).
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