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September 26, 2010
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概要
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癌症干细胞（CSCS）已确定在一些恶性肿瘤。在这个协议中，我们描述了一个流式细胞仪方法，利用醛脱氢酶活性和CD44和CD24的表达，从人胰腺癌移植瘤的社区体育会隔离。然后可以使用这些活细胞功能和分析研究。
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IsolationStem CellsHumanPancreatic CancerXenograftsCSCsSelf-renewalDifferentiated ProgenyTumorImmunocompromised MiceBreast CancerCell Surface Antigen ExpressionCD44+CD24low/-ALDH ActivityPancreatic AdenocarcinomaCD133Tumorigenic PopulationsInvasive CellsProtocolViable Pancreatic CSCsLow-passage Human Xenografts
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Rasheed, Z., Wang, Q., Matsui, W. Isolation of Stem Cells from Human Pancreatic Cancer Xenografts. J. Vis. Exp. (43), e2169, doi:10.3791/2169 (2010).
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