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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
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Multiplex Immunofluorescence Staining and Image Analysis
JoVE Journal
がん研究
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JoVE Journal がん研究
Multiplex Immunofluorescence Staining and Image Analysis

Multiplex Immunofluorescence Staining and Image Analysis

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:
10.3791/200119-v

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04:44 min

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June 02, 2023

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, , , , , , ,
1Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), 2Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), 3Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), 4IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), 5Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Multiplex ImmunofluorescenceDeparaffinizationEndogenous Peroxidase InhibitionCitrate BufferMicrowaveTris-buffered SalineTween 20BSAPrimary AntibodyPoly-HRP Secondary AntibodyTyramide ReagentPan-Cytokeratin AntibodyBisBenzimideFluorescent StainingImage Analysis

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Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Multiplex Immunofluorescence Staining and Image Analysis

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