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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
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Processing Murine Small Intestine for Crypt Isolation
JoVE Journal
生物学
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JoVE Journal 生物学
Processing Murine Small Intestine for Crypt Isolation

Processing Murine Small Intestine for Crypt Isolation

DOI:
10.3791/200006-v

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01:35 min

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June 02, 2023

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, ,
1Suntory Foundation for Life Sciences,Bioorganic Research Institute, 2Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, 3Faculty of Medicine,Osaka Medical and Pharmaceutical University

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Crypt IsolationSmall IntestineMurinePBS-antibioticsEuthanized AnimalManual WashingPipetteLaboratory ScissorsTweezersPetri Dish50-milliliter Tube

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