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Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues
JoVE Journal
生物学
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JoVE Journal 生物学
Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

Robotics and Dynamic Image Analysis for Studies of Gene Expression in Plant Tissues

DOI:
10.3791/1733-v

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11:26 min

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May 05, 2010

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, ,
1Department of Horticulture and Crop Science,The Ohio State University, 2Department of Plant Pathology,North Carolina State University

章

  • 00:00標題
  • 00:45Introduction
  • 01:34Gene Introduction Using Particle Bombardment
  • 04:51Automated Image Collection
  • 07:44Image Analysis
  • 09:14Representative Results of Gene Expression Imaging in Plant Tissues
  • 10:12Conclusion

概要

自動翻訳

自動翻訳

We report a method for introduction, tracking and quantitative analysis of GFP expression in plant cells. This method utilizes a custom-designed robotics system for semi-continuous image collection from large numbers of samples, over time. We also demonstrate the use of ImageJ and ImageReady for analysis of image series.

タグ

RoboticsDynamic Image AnalysisGene ExpressionGreen Fluorescent Protein (gfp)PromotersParticle BombardmentRobotic Image Collection SystemFluorescence Dissecting MicroscopeDigital CameraRobotics PlatformCulture DishesExpression ProfilesManual Image AlignmentImageReady SoftwareGFP Intensity MeasurementsImageJ SoftwareBackground Fluorescence

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