A Technique to Generate a Novel Delivery Platform for a Delayed Burst Release of Molecules
Published: May 31, 2024
Abstract
Source: Arun Kumar, S. et al., Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform. J. Vis. Exp. (2020)
This video demonstrates a method for the generation of a polymer bubble or polybubble-based novel platform that enables delayed burst release. Polyester-based polymers are used to form the polybubbles with core-shell structure, and small molecules and antigens can be used as cargo.
Protocol
1. Polycaprolactone triacrylate (PCLTA) synthesis Dry 3.2 mL of 400 Da polycaprolactone (PCL) triol overnight at 50 °C in an open 200 mL round bottom flask and potassium carbonate (K2CO3) in a glass vial at 90 °C. Mix the triol with 6.4 mL of dichloromethane (DCM) and 4.246 g of K2CO3 under argon. Mix 2.72 mL of acryloyl chloride in 27.2 mL of DCM and add dropwise to the reaction mixture in the flask over 5 min. <…
開示
The authors have nothing to disclose.
Materials
| 1-Step Ultra Tetramethylbezidine (TMB)-Enzyme-Linked Immunosorbent Assay (ELISA) Substrate Solution | Thermo scientific | 34028 | |
| 2-Hydroxy-2-methylpropiophenone | TCI AMERICA | H0991 | |
| 450 nm Stop Solution for TMB Substrate | Abcam | ab17152 | |
| Acryloyl chloride | Sigma Aldrich | A24109-100G | |
| Acriflavine | Chem-Impex International | 22916 | |
| Anhydrous ethyl ether | Fisher Chemical | E138-500 | |
| Anti-HIV1 gp120 antibody conjugated to horseradish peroxidase (HRP) | |||
| Bovine serum albumin (BSA) | Fisher BioReagents | BP9700100 | |
| BSA-CF488 dye conjugates | Invitrogen | A13100 | |
| Carboxymethylcellulose (CMC) | Millipore Sigma | 80502-040 | |
| Chloroform | Fisher Chemical | C2984 | |
| Coating buffer | Abcam | ab210899 | |
| Dichloromethane (DCM) | Sigma Aldrich | 270997-1L | |
| Diethyl ether | Fisher Chemical | E1384 | |
| Legato 100 Syringe Pump | KD Scientific | 14 831 212 | |
| Nonfat dry milk | Andwin Scientific | NC9022655 | |
| Potassium carbonate | Acros Organics | AC424081000 | |
| Phosphate saline buffer | Fisher BioReagents | BP3991 | |
| (Poly(caprolactone) | Sigma Aldrich | 440744-250G | |
| (Poly(caprolactone) triol | Acros Organics | AC190730250 | |
| Poly (lactic-co-glycolic acid) diacrylate | CMTec | 280050 | |
| Recombinant HIV1 gp120 + gp41 protein | Abcam | ab49054 | |
| Trehalose | Acros Organics | NC9022655 |
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