A Mouse Model of Vascularized Heterotopic Spleen Transplantation
Abstract
Source: Wang, J., et al. A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity. J. Vis. Exp. (2019).
The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen, the incision is closed, and the mouse is monitored for recovery.
Protocol
Representative Results
開示
The authors have nothing to disclose.
Materials
| Ketamine | Wyeth | 206205-01 | |
| Xylazine | Lloyd Laboratories | 139-236 | |
| Heparin solution | Abraxis Pharmaceutical Products | 504031 | |
| Injection grade normal saline | Hospira Inc. | NDC 0409-4888-20 | |
| 70% Ethanol | Pharmco Products Inc. | 111000140 | |
| ThermoCare Small Animal ICU System | Thermocare, Inc. | ||
| Adson Forceps | Roboz Surgical Instruments | RS-5230 | |
| Derf Needle Holder | Roboz Surgical Instruments | RS-7822 | |
| Extra Fine Micro Dissecting Scissors | Roboz Surgical Instruments | RS-5881 | |
| Micro-clip | Roboz Surgical Instruments | RS-5420 | |
| 7-0 silk | Braintree Scientific | SUT-S 103 | |
| 11-0 nylon on 4-mm (3/8) needle | Sharpoint DR4 | AK-2119 | |
| Ms CD45.2 antibody | BD Bioscience | 553772 | |
| Ms CD45.1 antibody | BD Bioscience | 553776 | |
| Ms CD11b antibody | BD Bioscience | 557657 | |
| Ms B220 antibody | BD Bioscience | 553089 | |
| Ms Ly6C antibody | eBioscience | 48-5932-80 | |
| Ms Ly6G antibody | BD Bioscience | 561236 | |
| Ms F4/80 antibody | BD Bioscience | 565614 | |
| Ms CD11c antibody | BD Bioscience | 558079 | |
| Ms CD3 antibody | eBioscience | 48-0032-82 | |
| Ms CD4 antibody | BD Bioscience | 552051 | |
| Ms CD8 antibody | BD Bioscience | 563786 | |
| LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit | Thermo Fisher | L34955 |
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