Intracranial Cannular Implantation in a Mouse to Deliver Therapeutic Agents to Brain Tumors

Published: May 31, 2024

Abstract

Source: Harvey, K. et al., Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice. J. Vis. Exp. (2023)

This video presents a procedure for intracranial cannula implantation in a brain tumor mouse model, to facilitate the administration of an immunotherapeutic agent. The delivery of glypican-2-specific chimeric antigen receptor T cells in the tumor microenvironment triggers T cell activation, resulting in tumor lysis and tumor regression.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparing the mouse for surgery Anesthetize the mouse in an induction chamber with isoflurane (2-4%) at an oxygen flow rate of 1 L/min until an adequate plane of anesthesia is reached (approximately 5 min). Weigh the mouse using a scale to the nearest 0.1 g and administer subcutaneous slow-release (SR) bupreno…

Representative Results

Figure 1: Guide cannula with projection dummy and treatment cannulas. (A) Guide cannula with 0.5 mm projection and 2 mm projection dummy cannulas in place. (B) Guide cannula with 0.5 mm projection and 2 mm projection treatment cannulas in place.

開示

The authors have nothing to disclose.

Materials

18 G needles BD 511097 1 1/2 inch metal hub
Acrylic resin liquid Lang Dental B1323
Acrylic resin powder Lang Dental B1323
Alcohol wipes BD 326895
Centrifuge 5240 Eppendorf 5420000040 Centrifuge
Cotton tipped swabs Puritan 826-WC Handle Width = 2.11 mm (0.083), Head Width = 1.27 mm (0.050), Handle Length = 147.62 mm (5.812), Overall Length = 152.4 mm (6), Head Length = 12.7 mm (0.500)
Drill bit holder P1 Technologies DH-1 Drill bit holder for D56-D70
Drill bit P1 Technologies D58 1.07 mm
Dummy cannula P1 Technologies C315DCS-5/SPC Configuration: Small cap;  Length: Cut 5.00 mm below pedestal; Projection: 0.50 mm
Flat tip screwdriver P1 Technologies SD-80 Screwdriver
Graefe forceps Fine Science Tools 11051-10 Forceps
Guide cannula P1 Technologies C315GS-5/SPC Configuration: 5.00 mm pedestal height; Length: Cut 5.00 mm below pedestal
Hemostatic cotton pellets with racemic epinephrine Pascal 1151602
MOXI Z Mini automated cell counter Kit Moxi MXZ001 Cell counter
NOD scid gamma (NSG) mice Jackson Laboratory 5557 6 to 12-week-old males and females
Pasteur pipet VWR 14673-043
PKG tubing P1 Technologies C313CT Diameter: 0.58 mm x 1.27 mm
Porcelain 12 well plate Flinn Scientific AP6064
Povidone iodine Medline MDS093943
Scalpel World Precision Instrument 50-822-457 Disposable Scalpel, no.10, sterile, 10/box, Plastic Handle with 6" Ruler
Screws P1 Technologies 0-80 X 3/32 2.4 mm
Stereotaxic Frame David Kopf Instruments 940 Model 940 Small Animal Stereotaxic Instrument with Digital Display Console
Student fine scissors Fine Science Tools 91460-12 Scissors
Treatment cannula P1 Technologies C315IS-5/SPC 33GA; Configuration: Standard internal; Length: Cut 5.00 mm below pedestal; Projection: 0.50 mm
Treatment syringes Hamilton 87908 5 µL, Model 75 Cemented Needle Special (SN) Syringe, 75SN/22/0.5"/PT3
Vactrap XL Foxx Life Sciences 305-4401-FLS Vacuum System

タグ

This article has been published
Video Coming Soon
Keep me updated:

.

記事を引用
Intracranial Cannular Implantation in a Mouse to Deliver Therapeutic Agents to Brain Tumors. J. Vis. Exp. (Pending Publication), e22232, doi: (2024).

View Video