A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats
Published: October 31, 2023
Abstract
Source: Hou, A., et al. A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (2017).
This video demonstrates a technique to induce chronic experimental autoimmune dry eye disease in rats. Upon injecting an antigen-adjuvant emulsion containing autoantigens and foreign antigens into a rat at predefined intervals, chronic eye inflammation is induced in the immunized rat by injecting the same antigens into the ocular surface tissues.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Lacrimal Gland Extract NOTE: Rats were anesthetized with the intraperitoneal injection of ketamine (75 mg/kg) and xylazine (10 mg/kg). Proper anesthetization was confirmed by toe pinching and tail pinching. Ophthalmic gel was applied to the rat's eyes to prevent dryness …
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The authors have nothing to disclose.
Materials
| Reagents | |||
| Protease inhibitor cocktail | Sigma-Aldrich | P2714-1BTL | |
| Pierce BCA Protein Assay Kit | Thermal Scientific | 23227 | |
| Mycobacterium tuberculosis H37Ra | Becton, Dickinson and company | 231141 | |
| complete Freund's adjuvant | Becton, Dickinson and company | 231131 | |
| ovalbumin | Sigma-Aldrich | A5503-10G | |
| incomplete Freund's adjuvant | Sigma-Aldrich | F5506-6X10ML | |
| pertussis toxin | Sigma-Aldrich | P7208-50UG | |
| fluorescein sodium solution | Bausch & Lomb U.K Limited | NA | |
| Equipment | |||
| Sonicator | Sonics | Vibra-Cell | |
| Micro IV microscope | Phoenix Research Labs | NA |
