A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats

Published: October 31, 2023

Abstract

Source: Hou, A., et al. A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (2017).

This video demonstrates a technique to induce chronic experimental autoimmune dry eye disease in rats. Upon injecting an antigen-adjuvant emulsion containing autoantigens and foreign antigens into a rat at predefined intervals, chronic eye inflammation is induced in the immunized rat by injecting the same antigens into the ocular surface tissues.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Lacrimal Gland Extract NOTE: Rats were anesthetized with the intraperitoneal injection of ketamine (75 mg/kg) and xylazine (10 mg/kg). Proper anesthetization was confirmed by toe pinching and tail pinching. Ophthalmic gel was applied to the rat's eyes to prevent dryness …

開示

The authors have nothing to disclose.

Materials

Reagents
Protease inhibitor cocktail Sigma-Aldrich P2714-1BTL
Pierce BCA Protein Assay Kit Thermal Scientific 23227
Mycobacterium tuberculosis H37Ra Becton, Dickinson and company 231141
complete Freund's adjuvant Becton, Dickinson and company 231131
ovalbumin Sigma-Aldrich A5503-10G
incomplete Freund's adjuvant Sigma-Aldrich F5506-6X10ML
pertussis toxin Sigma-Aldrich P7208-50UG
fluorescein sodium solution Bausch & Lomb U.K Limited NA
Equipment
Sonicator Sonics Vibra-Cell
Micro IV microscope Phoenix Research Labs NA

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記事を引用
A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats. J. Vis. Exp. (Pending Publication), e21763, doi: (2023).

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