A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation
Published: October 31, 2023
Abstract
Source: Yao, Y., et al. Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice. J. Vis. Exp. (2016).
This video demonstrates an in vivo technique to assess a test antigen by inducing skin inflammation in a murine model. The test antigen—a hapten in an oil/acetone solution—is topically applied to the skin of the mouse ear, and inflammation of the skin is assessed after an adequate period.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Induction of Skin Inflammation in pIL1-DsRed Mice Anesthetize mouse by intraperitoneal (i.p.) injection of an anesthetic cocktail containing 100 mg/kg ketamine, 10 mg/kg xylazine, and 1 mg/kg acepromazine. Adequately anesthetized mice do not show hind paw withdrawal to toe pinch. Apply hair-removal cream to th…
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The authors have nothing to disclose.
Materials
| Ketamine hydrochloride | Hospira | NDC 0409-2051-05 | |
| Xylazine | LLOYD Laboratory | NADA #139-236 | |
| Acepromazine | Boehringer Ingelheim | ANADA 200-361 | |
| Hair-removal cream | Church & Dwight | ||
| Acetone | Fisher Scientific | A16P4 | |
| Oxazolone | Sigma-Aldrich | E0753 |
