Diffuse Correlation Spectroscopy to Assess Cerebral Blood Flow in a Mouse Model
Published: June 29, 2023
Abstract
Source: Brothers, R. O., et al. Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury. J. Vis. Exp. (2022)
In this video, we demonstrate the diffuse correlation spectroscopy technique to measure cerebral blood flow in a mouse model of mild traumatic brain injury.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. Assessment of cerebral blood flow with diffuse correlation spectroscopy DCS data acquisition Remove hair on the scalp. Because DCS works best in the absence of hair, it is necessary to remove fur on the head prior to the start of experiments. Typically, hair removal is done 1-3 days prior to …
Representative Results
Figure 1: Measurement of cerebral blood flow by Diffuse Correlation Spectroscopy. (A) An optical sensor is gently manually held over the right hemisphere to measure blood flow in an anesthetized mouse. (B) Representative sensor placement on the right hemisphere. The outline of the sensor is represented as a dashed black rectangle, and the location of the source and detector fiber…
開示
The authors have nothing to disclose.
Materials
| Depilatory cream | Amazon | Nair | |
| DiH2O | VWR | VWRL0200-1000 | |
| Hardware Autocorrelator Board | www.correlator.com | Flex05-8ch | |
| Isoflurane 250 mL | MED-VET INTERNATIONAL | RXISO-250 | |
| Kimwipe (11.2 x 21.3 cm) | VWR | 21905-026 | |
| Laboratory vortex mixer | VWR | 10153-838 | |
| LabView | National Instruments | LabVIEW | |
| Luminex 200, HTS, FLEXMAP 3D, or MAGPIX with xPONENT software | Luminex Corporation | ||
| R Programming Language | |||
| RStudio | www.rstudio.com | ||
| Sonicator | |||
| 1 m acrylic guide tube | McMaster-Carr | 49035K85 | |
| 4 photon counting avalanche photodiode | Perkin-Elmer | SPCM-AQ4C-IO | |
| 400 um multimode source fiber | Thorlabs Inc. | FT-400-EMT | |
| 54 g bolt | Ace Hardware | 0.95 cm basic body diameter, 2 cm head diameter, 10.2 cm length | |
| 780 nm single mode detector fiber | Thorlabs Inc. | 780HP | |
| 852 nm long-coherence length laser | TOPTICA Photonics | iBeam smart |
