Recombinant Adeno Associated Virus Based Gene Delivery: A Technique for Transgene Delivery and Cardiac-Specific Expression in Rodent Model
Published: April 30, 2023
Abstract
Source: Ding, J. et al. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (2016)
In this video, we demonstrate recombinant adeno associated virus-based gene delivery in the mouse pup. This method helps in studying gene expression in the targeted cells.
Protocol
1. Treat neonatal mice with rAAV9 at P0.5-P2.5 by subcutaneous injection. Pre-fill a 29G1/2, 0.33 x 12.7 mm insulin syringe with the recombinant adeno-associated virus 9 (rAAV9) solution. Be careful to remove air bubbles. Hold the cryo-anesthetized pup in one hand with thumb and forefinger. Prior to injection, swipe the back skin of the pup with a swab stick saturated with 70% isopropyl alcohol to maintain the sterile condition. Insert the syringe needle into the anterior-dorsa…
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The authors have nothing to disclose.
