Assessing Vessel Perfusion via Angiography: A Technique to Introduce Contrast Agent in Descending Aorta to Visualize Blood Vessel Perfusion in Rabbit Model

Published: April 30, 2023

Abstract

Source: Sligar, A. D et al., Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).

This video describes the technique for performing angiography by injecting a contrast agent into the descending aorta to visualize blood vessel perfusion in a rabbit model. This technique helps to understand the perfusion in blood vessels with blockages and after treatment during recovery.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of rabbit for surgery Anesthetize the rabbit using 20–40 mg/kg ketamine and 2 mg/kg midazolam via subcutaneous injection. Place the rabbit on 1.5%–3% isoflurane (typically 2%) throughout the initial sedation using a mask. Give an injection of alfaxalone to maintain anesthesia via an intramuscular…

Representative Results

Figure 1: Angiograms for the hind limb of a diabetic and non-diabetic rabbit pre-surgery, post-surgery and after recovery for 70 days after femoral artery ligation and excision. (A) Angiogram of ischemic limb (left) and contralateral control limb (right). (B) Enlarged image of the ischemic limb at the site of ligation. 

開示

The authors have nothing to disclose.

Materials

Angiography Equipment   Toshiba Infinix-i
Guidewire  Boston Scientific  39122-01
Insertion Tool    Merit Medical Systems MAP550 metal wire insertion tool
Lidocaine   McKesson Medical-Surgical 239936
Ligaloop   V. Mueller  CH117 / CH116 White Mini / Yellow Mini
Pigtail Catheter   Merit Medical Systems 1310-21-0053  3F pigtail
Silk Sutures  Ethicon-Johnson & Johnson  A183H  4-0 silk ties 18"
Syringe Pump  DRE Veterinary  Versaflow VF-300
Visipaque contrast media  McKesson Medical-Surgical 509055
Angiography Injector  Medrad

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記事を引用
Assessing Vessel Perfusion via Angiography: A Technique to Introduce Contrast Agent in Descending Aorta to Visualize Blood Vessel Perfusion in Rabbit Model. J. Vis. Exp. (Pending Publication), e20927, doi: (2023).

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