Assessing Vessel Perfusion via Angiography: A Technique to Introduce Contrast Agent in Descending Aorta to Visualize Blood Vessel Perfusion in Rabbit Model
Published: April 30, 2023
Abstract
Source: Sligar, A. D et al., Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).
This video describes the technique for performing angiography by injecting a contrast agent into the descending aorta to visualize blood vessel perfusion in a rabbit model. This technique helps to understand the perfusion in blood vessels with blockages and after treatment during recovery.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of rabbit for surgery Anesthetize the rabbit using 20–40 mg/kg ketamine and 2 mg/kg midazolam via subcutaneous injection. Place the rabbit on 1.5%–3% isoflurane (typically 2%) throughout the initial sedation using a mask. Give an injection of alfaxalone to maintain anesthesia via an intramuscular…
Representative Results
Figure 1: Angiograms for the hind limb of a diabetic and non-diabetic rabbit pre-surgery, post-surgery and after recovery for 70 days after femoral artery ligation and excision. (A) Angiogram of ischemic limb (left) and contralateral control limb (right). (B) Enlarged image of the ischemic limb at the site of ligation.
開示
The authors have nothing to disclose.
Materials
| Angiography Equipment | Toshiba | Infinix-i | |
| Guidewire | Boston Scientific | 39122-01 | |
| Insertion Tool | Merit Medical Systems | MAP550 | metal wire insertion tool |
| Lidocaine | McKesson Medical-Surgical | 239936 | |
| Ligaloop | V. Mueller | CH117 / CH116 | White Mini / Yellow Mini |
| Pigtail Catheter | Merit Medical Systems | 1310-21-0053 | 3F pigtail |
| Silk Sutures | Ethicon-Johnson & Johnson | A183H | 4-0 silk ties 18" |
| Syringe Pump | DRE Veterinary | Versaflow VF-300 | |
| Visipaque contrast media | McKesson Medical-Surgical | 509055 | |
| Angiography Injector | Medrad |
