Hepatic Angiography: A Procedure to Visualize Liver Tumor Vessels in a Rabbit Model
Published: April 30, 2023
Abstract
Source: Khabbaz, R. C., et al. Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer. J. Vis. Exp. (2019).
In this video, we demonstrate a step-by-step procedure to perform hepatic angiography using a fluoroscope that enables the real-time visualization of blood vessels associated with a liver tumor in a rabbit model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Angiographic Utilization of the VX2 Liver Tumor Anesthetize the rabbit using 45 mg/kg ketamine and 5 mg/kg xylazine for induction followed by intubation and maintenance with 1%-3% isoflurane as needed. When the rabbit is anesthetized, shave the surgical site using hair clippers. Clean the incision area using a triplicate wash with betad…
Representative Results
Figure 1: The femoral groove and initial incision. (A) Palpation of the hind limb allows for visualization of the femoral groove (white dotted line). (B) Initial incision in the hind limb made along the femoral groove. Fi…
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The authors have nothing to disclose.
Materials
| Hair Clippers | Wahl | 41870-0438 | |
| Ketamine | Henry Schein | 56344 | |
| Xylazine | Akorn | 59399-110-20 | |
| 15-Blade | Steris | 02-050-015 | |
| 11-Blade | Steris | 02-050-011 | |
| Scalpel Handle x 2 | Steris | 22-2381 | |
| 3-0 PDS / Taper | Ethicon | Z305H | |
| 4 – 0 Vicryl / Cutting | Ethicon | J392H | |
| 3-F VSI Micro-HV Introducer Kit | Vascular Solutions | Custom Order (P15180391) | |
| .018 45° angle glidewire | Terumo | RG*GA1818SA | |
| Direxion bern-shape microcatheter | Boston Scientific | M001195230 | |
| Omnipaque | GE | Y510 |
