Hepatic Angiography: A Procedure to Visualize Liver Tumor Vessels in a Rabbit Model

Published: April 30, 2023

Abstract

Source: Khabbaz, R. C., et al. Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer. J. Vis. Exp. (2019).

In this video, we demonstrate a step-by-step procedure to perform hepatic angiography using a fluoroscope that enables the real-time visualization of blood vessels associated with a liver tumor in a rabbit model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Angiographic Utilization of the VX2 Liver Tumor Anesthetize the rabbit using 45 mg/kg ketamine and 5 mg/kg xylazine for induction followed by intubation and maintenance with 1%-3% isoflurane as needed. When the rabbit is anesthetized, shave the surgical site using hair clippers. Clean the incision area using a triplicate wash with betad…

Representative Results

Figure 1: The femoral groove and initial incision. (A) Palpation of the hind limb allows for visualization of the femoral groove (white dotted line). (B) Initial incision in the hind limb made along the femoral groove. Fi…

開示

The authors have nothing to disclose.

Materials

Hair Clippers Wahl 41870-0438
Ketamine Henry Schein 56344
Xylazine Akorn 59399-110-20
15-Blade Steris 02-050-015
11-Blade Steris 02-050-011
Scalpel Handle x 2  Steris  22-2381
3-0 PDS / Taper  Ethicon  Z305H
4 – 0 Vicryl / Cutting  Ethicon  J392H
3-F VSI Micro-HV Introducer Kit  Vascular Solutions  Custom Order (P15180391)
.018 45° angle glidewire  Terumo  RG*GA1818SA
Direxion bern-shape microcatheter  Boston Scientific  M001195230
Omnipaque  GE  Y510

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記事を引用
Hepatic Angiography: A Procedure to Visualize Liver Tumor Vessels in a Rabbit Model. J. Vis. Exp. (Pending Publication), e20920, doi: (2023).

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