Cardiopulmonary Block Harvest from Rabbit: A Surgical Procedure to Retrieve Functional Heart-Lung Block from Rabbit Model

Published: April 30, 2023

Abstract

Source: Pacheco-Baltazar, A., et al. Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021)

In this video, we show a procedure to surgically harvest a functional cardiopulmonary block from a rabbit model. The harvested block can be used for further physiological, pharmacological or biochemical studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Equipment and preparation of apparatus. Equipment arrangement: Set up an operating table with size according to the weight of the rabbit. Mount the cover of the artificial thorax on the steel column with the glass chamber underneath and the ventilator with a roller pump by the sid…

開示

The authors have nothing to disclose.

Materials

2-Stop Tygon E-Lab Tubing, 3.17 mm ID, 12/pack, Black/White Hugo Sachs Elektronik (HSE) 73-1864
Adapter for Positive Pressure Ventilation on IPL-4 Hugo Sachs Elektronik (HSE) 73-4312
Alternative Pressure-Free Gas Supply for IPL-4: To supply the trachea with gas mixture different from room air during negative ventilation Hugo Sachs Elektronik (HSE) 73-4309
Base Unit for the Rabbit to Fetal Pig Isolated Perfused Lung Hugo Sachs Elektronik (HSE) 73-4138
Bovine serum A2:D41albumin lyophilized powder sigma 3912 500 g
Differential Low Pressure Transducer DLP2.5, Range +- 2.5 cmH2O, HSE Connector Hugo Sachs Elektronik (HSE) 73-3882
Differential Pressure Transducer MPX, Range +- 100 cmH2O, HSE Connector Hugo Sachs Elektronik (HSE) 73-0064
Ethanol absolute HPLC grade Caledon
Harvard Peristaltic Pump P-230 (Complete with Control Box and P-230 Motor Drive) Hugo Sachs Elektronik (HSE) 70-7001
Heated Linear Pneumotachometer 0 to 10 L/min flow range Hugo Sachs Elektronik (HSE) 59-9349
Heater Controller for Single Pneumotachometer 230 VAC, 50 Hz Hugo Sachs Elektronik (HSE) 59-9703
Heparin PISA 5000 UI
IPL-4 Core System for Isolated Rabbit to Fetal Pig Lung, 230 V Hugo Sachs Elektronik (HSE) 73-4296
Lauda Thermostatic Circulator, Type E-103, 230 V/50 Hz, 3 L Bath Volume, Temperature Range 20 to 150°C Hugo Sachs Elektronik (HSE) 73-0125
Negative Pressure Ventilation Control Option with Pressure Regulator for IPL-4 Hugo Sachs Elektronik (HSE) 73-4298
New Zeland rabbits
PISABENTAL (Pentobarbital sodium) PISA Q-7833-215
PLUGSYS Case, Type 603* 7 Hugo Sachs Elektronik (HSE) 73-0045
PLUGSYS TCM Time Counter Module Hugo Sachs Elektronik (HSE) 73-1750
PLUGSYS Transducer Amplifier Module (TAM-A) Hugo Sachs Elektronik (HSE) 73-0065
PLUGSYS Transducer Amplifier Module (TAM-D) Hugo Sachs Elektronik (HSE) 73-1793
PLUGSYS VCM-4R Ventilation Control Module with Pressure Regulator Hugo Sachs Elektronik (HSE) 73-1755
PROCIN (Xylacine clorhydrate) PISA Q-7833-099
Pulmonary Artery Cannula for Rabbit with Basket, OD 4.6 mm Hugo Sachs Elektronik (HSE) 73-4161
Scalpel knife
Servo Controller for Perfusion (SCP) Hugo Sachs Elektronik (HSE) 73-2806
Surgical gloves No. 7 1/2
Surgical gloves No. 8
Tracheal Cannula for Rabbit, OD 5.0 mm Hugo Sachs Elektronik (HSE) 73-4163

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記事を引用
Cardiopulmonary Block Harvest from Rabbit: A Surgical Procedure to Retrieve Functional Heart-Lung Block from Rabbit Model. J. Vis. Exp. (Pending Publication), e20819, doi: (2023).

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