Intact Ovary Harvest from Mouse: A Surgical Procedure to Isolate Complete Ovary from Murine Model
Published: April 30, 2023
Abstract
Source: Mason, J. B. et al. Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice. J. Vis. Exp. (2018)
In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Transplantation of intact ovaries to aged mice NOTE: This protocol describes the procedure used during the transfer of ovaries from a young mouse (60 days of age) to a post reproductive female (12 months of age) of the same inbred strain. The same procedure is used for ovarian transplantat…
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The authors have nothing to disclose.
Materials
| Dissecting microscope | Any dissecting scope with an adjustable working distance. | ||
| Warm Water Recirculator and appropriate pad for your size surgical/recovery field. | Stryker/Gaymar | TP700 | Circulating water pads produce even and consistent temperature operating and recovery fields. |
| Surgical instruments-dissection instruments for access to the reproductive tract | Choice of specific surgical instruments is highly surgeonspecific | ||
| Suture material for ovarian bursa | eSutures | 2809G: 9-0 or 2810G: 10-0 ETHILON BLACK 5" BV130-5 TAPER | 10-0 suture can be difficult to locate if dropped on the surgery field. |
| Absorbable gelatin sponge | PFIZER | Gelfoam- PFIZER 09-0315-08 | cut into small (~2mm) pieces under sterile conditions. |
| Staples for skin closure | Becton Dickinson | MikRon Autoclip 9mm (BD 427631) | |
| Eye ointment. | MWI Veterinary Supply Co. | Puralube sterile opthalmic ointment | |
| Syringes for anesthesia, analgesia and epinephrine | MWI Veterinary Supply Co | BD 305536 | 27g x 3/8in,Intradermal Bevel 1/2ml |
| Syringes for saline. | MWI Veterinary Supply Co | BD 309657. | 3ml syringe, luer-lok tip, 27g needle |
| Clippers/blade | Andis | Super AGR+™ Cordless Detachable Blade Clipper and a CeramicEdge® #40 blade. | Any clippers with a fine blade. |
| Ethanol | University chemical supply | dilute to 70% for use. | |
| Betadine solution | MWI Veterinary Supply Co. | 3233 | |
| 2×2 gauze pads | MWI Veterinary Supply Co. | 3073 | autoclaved with surgery packs |
| Borosilicate glass beads | Sigma-Aldrich Co | Z273619 | Autoclave in sintilation vials |
| 4-vinylcyclohexene diepoxide | Sigma-Aldrich Co. | 94956 |
