Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding

Published: April 30, 2023

Abstract

Source: Gou, W. et al. Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice. J. Vis. Exp. (2021)

In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.

Protocol

1. Islet transplantation Induce and maintain the mouse under general anesthesia with 2% isoflurane. Check for the lack of pedal reflexes to ensure proper anesthetization of the animal. Shave and remove the fur in the abdomen area of the mouse. Administer a single pre-operative dose of Buprenorphine (0.1 mg/kg i.p.). Disinfect the surgical area with three alternating wipes of 2% iodine and 75% alcohol. Perform a laparotomy with micro scissors to generate…

Representative Results

Figure 1: Illustration of intrahepatic islet transplantation procedures. (A, C). Schematics of key steps used in Method A and Method B. (B, D). Islets were injected directly via the portal vein (C) or indirectly via fat pat (D).

開示

The authors have nothing to disclose.

Materials

10% Neutral buffered formalin v/v  Fisher Scientific  23426796
1 mL Syringe with needle  AHS  AH01T
20 mL Syringe  BD  301031
25G x 5/8" hypodermic needles  BD  305122
Alcohol prep pads, sterile  Fisher Scientific  22-363-750
Animal Anesthesia system  VetEquip, Inc.  Inc. 901806
Buprenorphine hydrochloride, injection  Par Sterile Products LLC  NDC 42023-179-05
Ethanol absolute (200 proof), molecular biology grade  Fisher Scientific  BP2818500
Extra fine Micro Dissecting scissors 4” straight sharp  Roboz Surgical Instrument Co.  RS-5882
Gelfoam (absorbable gelatin sponge USP)  Pharmacia & Upjohn Company  34201
Graefe forceps 4” extra delicate tip  Roboz Surgical Instrument Co.  RS-5136
Heated pad  Amazon  B07HMKMBKM
Hegar-Baumgartner Needle Holder 5.25”  Roboz Surgical Instrument Co.  RS-7850
Insulin syringe with 27-gauge needle  BD  879588
Iodine prep pads  Fisher Scientific  19-027048
Isoflurane  Piramal Critical Care  NDC 66794-017-25
Polypropylene Suture 4-0  Med-Vet International  MV-8683
Polypropylene Suture 5-0  Med-Vet International  MV-8661
Sodium chloride,  0.9% intravenous solution  VWR  2B1322Q
Surgical drape, sterile  Med-Vet International  DR1826

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記事を引用
Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding. J. Vis. Exp. (Pending Publication), e20790, doi: (2023).

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