Exertional Heat Stroke Mouse Model: A Protocol to Study Mechanisms Underlying Exertional Heat Stroke
Published: April 30, 2023
Abstract
Source: King, M. A. et al. A Preclinical Model of Exertional Heat Stroke in Mice. J. Vis. Exp. (2021).
In this video we describe a step-by-step protocol to study exertional heat stroke (EHS) in a mouse model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. EHS Protocol The night before the EHS protocol, place the mouse in the environmental chamber at room temperature (~25 °C, ≈30% relative humidity) to acclimate to the chamber. Use a data acquisition system to collect continuous core temperature (Tc), averaged over 30 s intervals overnight. …
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The authors have nothing to disclose.
Materials
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| BK Precision Multi-Range Programmable DC Power Supplies Model 9201 | BK Precision | ||
| Layfayette Motorized Wheel Model #80840B | Layfayette | ||
| Scientific Environmental Chamber Model 3911 | ThermoForma | ||
| Training Wheels | Columbus Inst | ||
| Energizer Power Supply | Starr Life Sciences | ||
| G2 Emitter et al. | Starr Life Sciences | ||
| Platform receiver et al. | Starr Life Sciences |
