Dissection of Palate Tissue from Adult Murine Model: A Surgical Procedure to Harvest Hard Palate and Soft Palate from Oral Cavity of Adult Mouse
Published: April 30, 2023
Abstract
Source: Xuan Ngo, Y. et al. Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate. J. Vis. Exp. (2021)
This video demonstrates a surgical procedure to dissect the palate tissue from a murine model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissection of Palate Tissue from Adult Mouse Sacrifice an adult C57BL/6J mouse (either male or female) by cervical dislocation in compliance with the facility's regulations relating to animal welfare. Remove the hair around the mouth with a shaver. Using scissors, cut from the cheek towa…
Representative Results
Figure 1: Overview of the dissection procedure and isolation of mouse oral keratinocytes. (A) Schematic representation of the mouse oral cavity. (B) Instruments used to dissect the palate and isolate mouse oral keratinocytes. (C) Brightfield image of the mouse palate. Scale bar: 100 µm. (D) Summary of th…
開示
The authors have nothing to disclose.
Materials
| Iris Scissors | Muromachi Kikai | 14090-09 | |
| Medetomidine | Nippon Zenyaku Kogyo | Domitor | |
| Midazolam | Astellas Pharma | Dormicum | |
| No.15 Disposable scalpel | Feather | 219AABZX00136000 | |
| Butorphanol | Meiji Seika Pharma | Vetorphale | |
| Half-curved forceps | BRC Bio Research Center | PRI13-3376 | |
| Povidone-iodine | Y's Square | 872612 | |
| Raspatorium #14 | AS ONE | 8-4599-01 | |
| Fine forceps | BRC Bio Research Center | PRI13-3374 | |
| Antibiotic-Antimycotic 100x | Gibco | 15240062 | |
| 15 mL sterile conical tube | Falcon | 352096 | |
| Cotton | AS ONE | 63-1452-97 | |
| EpiLife Defined Growth Supplement (EDGS) | Gibco | S0120 |
