Modeling Cancerous Neural Invasion In Vitro: A Method to Co-culture Dorsal Root Ganglia and Cancer Cells
Published: April 30, 2023
Abstract
Source: Na'ara, S. et al. In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model. J. Vis. Exp. (2016)
In this video, we demonstrate an in vitro method for co-culturing DRG and cancer cells to study neurotropism and neural invasion.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Isolating the Dorsal Root Ganglia (DRG) Use a stereomicroscope with 4X magnification. Position the spine on a non-adherent, non-absorbing platform (Telfa/nylon). Position the spine face up at the same craniocaudal orientation. Remove any spinal muscles and connective tissue. Use …
Representative Results
Figure 1: Schematic illustration of the protocol steps.
開示
The authors have nothing to disclose.
Materials
| Operating microscope | Leica | M205 | |
| Forceps | Sigma-Aldrich | F4142 | |
| Surgical blade | Sigma-Aldrich | Z309036 | |
| Scissors | Sigma-Aldrich | S3271 | |
| 35 mm Petri dishes glass bottom | de groot | 60-627860 | |
| 70% ethanol | Sigma | ||
| Cold PBS | Biological industries | 02-023-1A | |
| DMEM | Biological industries | 01-055-1A | |
| FCS | Rhenium | 10108165 | |
| Penicillin and streptomycin | Biological industries | 01-031-1B | |
| Sodium Pyruvate | Biological industries | 03-042-1B | |
| L-Glutamine | Biological industries | 03-020-1B | |
| Growth factor-depleted matrigel | Trevigen | 3433-005-01 |
