Intramucosal Inoculation of Cancer Cells in Murine Model: A Procedure to Establish an Orthotopic Head and Neck Cancer Mouse Model
Published: April 30, 2023
Abstract
Source: Oweida, A. J. et al. Intramucosal inoculation of squamous cell carcinoma cells in mice for tumor immune profiling and treatment response assessment. J. Vis. Exp. (2019)
In this video, we develop an orthotopic head and neck cancer murine model by intramucosal inoculation of the squamous cell carcinoma cell line. The tumor-bearing mouse would show resemblance to clinically relevant symptoms of human disease and can thus be used to study cancer progression and discover novel therapeutic strategies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Cell Injection into Mice Prepare a 1:1 mixture of cells:basement membrane matrix (50 µL each). Add the cells first; then, gradually pipette the basement membrane matrix. Avoid introducing air bubbles. Ensure that the mixture is made immediately before the animal injection. Adding cells to the basement mem…
開示
The authors have nothing to disclose.
Materials
| HBSS | ThermoFisher Scientific | 14175079 | No calcium, no magnesium, no pheno red |
| Helois mass cytometer | Fluidigm | NA | |
| Matrigel membrane matrix | Corning | CB-40234B |
