Elastic Fiber Staining of Slide Sections: A Technique to Observe Gastric Cancer Metastasis

Published: April 30, 2023

Abstract

Source: Lei, G. et al. Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue. J. Vis. Exp. (2019)

In this video, we demonstrate the elastic staining of sections obtained from gastrointestinal tumor tissue. This staining procedure enables visualization of fine elastic fibers and helps determine tumor cell invasion beyond the elastic lamina.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Staining Before proceeding with staining, wipe off any excess solutions around the tissue on the slide with tissue paper. Place the slides in room temperature and room humidity conditions to avoid drying of the tissue on the …

開示

The authors have nothing to disclose.

Materials

Elastin-Van Gieson staining kit Baso BA4083B Used for staining of elastic fibers, collagen fibers and myofibers
Ethanol LMAI Bio LM64-17-5 100%

タグ

Play Video

記事を引用
Elastic Fiber Staining of Slide Sections: A Technique to Observe Gastric Cancer Metastasis. J. Vis. Exp. (Pending Publication), e20411, doi: (2023).

View Video