Obtaining Primary Ovarian Cancer Cells from Solid Specimens: A Method to Culture Epithelial Ovarian Cancer Cells from Ovarian Tumor Specimens

Published: April 30, 2023

Abstract

Source: Pribyl, L. J. et al. Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens. J. Vis. Exp. (2014)

In this video, we describe a method for isolating and characterizing primary ovarian cancer cells from solid ovarian tumor tissue specimens. The epithelial cancer cells obtained using this protocol are suitable for understanding the processes that may lead to ovarian cancer initiation and development.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Tissue Processing Working under sterile conditions, transfer the samples onto a Petri dish (60 mm x 60 mm) containing 10 ml of fresh, ice-cold PBS and using a sterile razor blade, further cut into the smallest pieces possible (2 mm or less) (<…

Representative Results

Figure 1. Processing of clinical specimens. A) Solid specimen transferred onto a Petri dish containing 10 ml of fresh, ice-cold 1x PBS. B) Clinical specimens further cut into pieces about 2 mm in size Figure 2. S…

開示

The authors have nothing to disclose.

Materials

1x PBS, sterile Invitrogen 14190-144
Screw lid, polypropylene specimen
container, sterile
Thermo Scientific
DMEM medium, sterile Invitrogen 11995-065
Fetal bovine serum, sterile Thermo Scientific Hyclone SH30396.03
100x Penicillin-streptomycin, liquid Invitrogen 15140-122
Dispase II, sterile Roche 04942 078 001
Small fine-tip forceps and scalpel
blade, sterile
Fisher Scientific forceps: 08-953E, blades:
08-927-5D
Small dissecting scissors, sterile Fisher Scientific 08-945
15 ml Conical capped tubes, sterile BD Falcon 352097
50 ml Conical capped tubes, sterile BD Falcon 352027
10 ml Serological pipette, sterile Corning 13-678-11E
6 ml Syringe plunger, sterile Tyco Healthcare 8881516911
Nylon filter (70 μm ) cell strainer,
sterile
BD Falcon 352350
5 cm Petri dish, sterile Corning 430166
10 cm Petri dish, sterile Corning 430167

タグ

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記事を引用
Obtaining Primary Ovarian Cancer Cells from Solid Specimens: A Method to Culture Epithelial Ovarian Cancer Cells from Ovarian Tumor Specimens. J. Vis. Exp. (Pending Publication), e20381, doi: (2023).

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